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IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression
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Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Marca:  IBA Lifesciences 5-4543-001

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Codice prodotto. 15653798

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Descrizione

Descrizione

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

  • NeoR for generation of stable cells lines.
  • Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
  • SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
  • ColE1 + Amp: for propagation in E. coli
  • NeoR: neomycin resistance gene to generate stable cell lines
  • BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifica

Specifica

Liquid
SV40 large T antigen (e.g. COS1 or COS7)
8
Acceptor Vector for mammalian expression
2°C to 8°C for frequent usage, -20°C for long-term storage
250 ng/μL
pESG-IBA 143
Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins
StarGate™ System, Mammalian Host Cells
Neomycin resistance gene
Human CMV Immediate-early
20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
His Tag (6x) for N-terminal and Twin-Strep-tag for C-terminal
5 μg
Clear
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Titolo del prodotto
IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression > StarGate pESG Acceptor Vector: pESG-IBA 143; 6xHistidine-tag for N-terminal and Twin-Strep-tag for C-terminal; Includes Neomycin resistance gene

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