IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression

Designed for high-level constitutive expression of recombinant proteins in a wide range of Mammalian host cells through the CMV promoter, with replication in cell lines that are latently infected with SV40 large T antigen.

Marque: IBA Lifesciences 5-4545-001

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Code produit. 15663798

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Description

pESG-IBA mammalian expression vectors

pESG-IBA is a small expression vector recommended for transient expression of proteins in cell lines providing SV40 large T-antigen for extrachromosomal replication with SV40 ori.

NeoR for generation of stable cells lines.
Cytomegalovirus immediate-early (CMV) promoter: high level constitutive expression
SV40 ori: origin of replication for extrachromosomal replication driven by SV40 large T-antigen (as provided by HEK293T or COS-1, -3, -7 cells)
ColE1 + Amp: for propagation in E. coli
NeoR: neomycin resistance gene to generate stable cell lines
BM40: secretion signal to transfer the expressed protein into the medium (not for all vectors available)

Please note that cloning into pESG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Spécification

Formato	Liquid
Agente inducente	SV40 large T antigen (e.g. COS1 or COS7)
pH	8
Product Type	Acceptor Vector for mammalian expression
Content And Storage	2°C to 8°C for frequent usage, -20°C for long-term storage
Concentrazione	250 ng/μL
Vettore	pESG-IBA 145
Da utilizzare con (applicazione)	Cloning Genes of Interest for Use in Functional Expression, Purification, Detection, Immobilization or Separation of Recombinant Proteins

Da utilizzare con (apparecchiatura)	StarGate™ System, Mammalian Host Cells
Include	Neomycin resistance gene
Promotore	Human CMV Immediate-early
Tampone di conservazione	20 μL TE buffer: 10 mm Tris-HCl, 1 mm EDTA / 5 μg
Protein Tag	Twin-Strep-tag for N-terminal and His Tag (6x) for C-terminal
Quantità	5 μg
Colore	Clear

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Documents

Product Information

Manuals

Manual StarGate 5-4545-001 Dimensione file: 2.3 MB

Manual StarGate-Direct-Transfer-Cloning 5-4545-001 Dimensione file: 1.7 MB

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Nom du produit

IBA Lifesciences StarGate pESG-IBA Vectors for Mammalian Expression > StarGate pESG Acceptor Vector: pESG-IBA 145; Twin-Strep-tag for N-terminal and 6xHistidine-tag for C-terminal; Includes Neomycin resistance gene

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