Tyrosinase Ab-1 Mouse Monoclonal Antibody, Epredia™

Descrizione

Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.

Host Species: Mouse

Clone: T311

Isotype: IgG2a

Species Reactivity: Human, Dog and Cat. Weakly cross-reacts with Mouse. Others not known.

Epitope: aa 1-433

Immunogen: Recombinant tyrosinase protein

Molecular Weight: 70-80kDa

Positive Control: Melanoma cell lines and Melanoma

Cellular Localization: Cytoplasmic Ab-1 shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Staining of melanomas with Ab-1 showed tyrosinase in melanotic as well as amelanotic variants. Ab-1 is a useful marker for melanocytes and melanomas. Occasionally a minor band at 55kDa is also detected in Western Blotting

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunofluorescence
• Immunocytology