Life Science Buffers

Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Compresse PBS, tampone fosfato salino, Fisher BioReagents
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Tamponi di uso comune
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Agente tamponante molto diffuso
Peso formulazione | 238.197 |
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Sinonimo | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 1M Solution, pH 7.3 |
Colore | Non definito |
Forma fisica | Liquido |
Conservazione consigliata | RT |
DNasi | Esente da DNasi |
Identificazione | Pass Test |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Formula molecolare | C8H18N2O4S |
CAS | 7732-18-5 |
Indice di Merck | 15, 4689 |
pH | 7.2 to 7.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | HEPES Buffer |
Grado | Biologia molecolare |
Concentrazione | 0.95 to 1.05 M |
Colore | White |
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Content And Storage | Room temperature |
Forma | Powder |
Tipo di imballaggio | Poly Bottle |
Soluzione di cloruro di potassio, 3M

Gli ordini eseguiti prima delle 14:00 saranno spediti oggi stesso.
Gli ordini eseguiti dopo le 14:00 saranno spediti domani.
Per saperne di più
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formula molecolare: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Sinonimo: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Sinonimo | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
---|---|
PubChem CID | 6503 |
Formula molecolare | C4H11NO3 |
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure, Thermo Scientific Chemicals
CAS: 1185-53-1 Formula molecolare: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PubChem CID | 93573 |
---|---|
Formula molecolare | C4H12ClNO3 |
CAS | 1185-53-1 |
Molecular Weight (g/mol) | 157.594 |
SMILES | C(C(CO)(CO)N)O.Cl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
Sinonimo | TAE |
---|---|
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 50X Solution |
Forma fisica | Liquid |
Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
CAS | 6850-28-8 |
Conservazione consigliata | RT |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Filtered Through | Filtered through a 0.2-micron filter. |
Grado | Elettroforesi |
DNasi | Esente da DNasi |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
Sinonimo | TE |
---|---|
Nota nome | 1X Solution, pH 8.0 |
Pericolo per la salute 2 | CAUTION! |
Colore | Colorless |
Pericolo per la salute 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Forma fisica | Liquid |
Conservazione consigliata | RT |
Filtered Through | Filtered through a 5-micron filter. |
DNasi | Esente da DNasi |
CAS | 7732-18-5 |
pH | 7.4 to 8.1 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | Tris-EDTA |
Grado | Biologia molecolare |
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent.
Numero MDL | MFCD00006158 |
---|---|
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Pericolo per la salute 2 | WARNING! |
Colore | White |
Pericolo per la salute 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
Conservazione consigliata | RT |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
DNasi | Esente da DNasi |
Identificazione | Pass Test |
PubChem CID | 23831 |
Intervallo percentuale saggio | ≥99 % |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
Formula molecolare | C8H18N2O4S |
Percent Purity | ≥99% |
Informazioni di solubilità | Soluble in water |
CAS | 7365-45-9 |
Indice di Merck | 15, 4689 |
pH | 5.0 to 6.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | HEPES |
Grado | Biologia molecolare |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Sinonimo: TBE
Sinonimo | TBE |
---|---|
CAS | 10043-35-3 |
TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade, Thermo Scientific Chemicals
CAS: 1185-53-1 Formula molecolare: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PubChem CID | 93573 |
---|---|
Formula molecolare | C4H12ClNO3 |
CAS | 1185-53-1 |
Molecular Weight (g/mol) | 157.594 |
SMILES | C(C(CO)(CO)N)O.Cl |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Punto di fusione | 0°C |
pH | 7.3 to 7.5 |
Tipo di imballaggio | Poly Cube |