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  8. 10121360

Thermo Scientific™ Trascrittasi inversa RevertAid H Minus

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Marca:  Thermo Scientific™ EP0451

135.10 EUR valido fino al 2024-12-20
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Codice prodotto. 10121360

  • 193.00€ / 10000 unità

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Descrizione

Descrizione

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).

Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

TRUSTED_SUSTAINABILITY
Specifica

Specifica

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.
60 min.
42°C to 45°C
RevertAid H Minus
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
Stand-alone Enzyme
Reverse Transcription
10,000 Units
Reduced
200 U/μL
Separate components
Up to 13 kb
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For Research Use Only. Not for use in diagnostic procedures.