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Thermo Scientific™ Trascrittasi inversa RevertAid H Minus
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
Marca: Thermo Scientific™ EP0451
134.00 EUR valido fino al 2025-03-21
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Descrizione
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).
Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)
Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Specifica
• RevertAid H Minus Reverse Transcriptase (200 U/μL) |
|
60 min. | |
42°C to 45°C | |
RevertAid H Minus | |
Dry Ice | |
First-Strand cDNA | |
Reverse Transcription | |
RNA |
Stand-alone Enzyme | |
Reverse Transcription | |
10,000 Units | |
Reduced | |
200 U/μL | |
Separate components | |
Up to 13 kb |
Suggerimenti di prodotto
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Certificati
Per poter ottenere i certificati è necessario indicare un numero di lotto. Per individuare il numero di lotto su ordini precedenti usi l'area dedicata allo stato degli ordini.
Numero di lotto | Tipo di certificato | Data | Catalog Number |
---|---|---|---|
2795748 | Certificato di analisi | 29/01/2025 | EP0451 |
2795731 | Certificato di analisi | 29/01/2025 | EP0451 |
2795754 | Certificato di analisi | 29/01/2025 | EP0451 |
2834957 | Certificato di analisi | 29/01/2025 | EP0451 |
2873433 | Certificato di analisi | 28/01/2025 | EP0451 |
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For Research Use Only. Not for use in diagnostic procedures.