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Thermo Scientific™ Agarosio HisPur™ Ni-NTA Superflow

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

180.00€ - 9260.00€

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Specifica

Capacità (sistema metrico) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Forma Liquid Suspension
Product Line HisPur™
Bersaglio His-Tagged Protein
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Prodotti 4
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
Codice del prodotto Marca Quantità Prezzo Quantità e disponibilità  
13414199
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Thermo Scientific™
25217 		1 L
9260.00€
1L
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13484189
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Thermo Scientific™
25214 		10 ml
180.00€
10mL
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Thermo Scientific™
25215 		50 ml
738.00€
50mL
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Thermo Scientific™
25216 		250 mL
2875.00€
250mL
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Descrizione

Descrizione

Perform FPLC purification of poly-histidine-tagged proteins with Thermo Scientific™ HisPur Ni-NTA Superflow Agarose, a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).


Additional Information and Online Ordering »

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Specifica

Specifica

20 mg His-Tagged GFP Binding per mL Resin
Liquid Suspension
His-Tagged Protein
Divalent Nickel, Metal Chelate
Affinity, Agarose Resin
Store at 4°C
HisPur™
Agarose
Bottle
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SDS
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Documenti

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.