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Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Marca:  Thermo Scientific™ F160S

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Codice prodotto. 15255156

  • 172.00€ / 100 reazioni

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Descrizione

Descrizione

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
TRUSTED_SUSTAINABILITY
Specifica

Specifica

• 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 1 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)

Store at -20°C.
Direct PCR
Fast
Direct PCR Master Mix
Wet or Dry Ice
2X
250 Reactions
SuperMix or Master Mix
DNA
Medium
Phire Hot Start II DNA Polymerase
Yes
250 reactions
Genotyping, Hot-start PCR
Built-In Hot Start
Blunt
Plant
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Numero di lotto Tipo di certificato Data Catalog Number
Numero di lotto3130092 Tipo di certificatoCertificato di analisi Data12/02/2025 Catalog NumberF160S
Numero di lotto2832652 Tipo di certificatoCertificato di analisi Data12/02/2025 Catalog NumberF160S
Numero di lotto3152046 Tipo di certificatoCertificato di analisi Data12/02/2025 Catalog NumberF160S
Numero di lotto2832632 Tipo di certificatoCertificato di analisi Data12/02/2025 Catalog NumberF160S
Numero di lotto2830563 Tipo di certificatoCertificato di analisi Data12/02/2025 Catalog NumberF160S
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Titolo del prodotto
Thermo Scientific™ Phire Plant Direct PCR Master Mix > 250 Reactions

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