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Thermo Scientific™ Miscela master per PCR Phire™ Tissue Direct
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Marca: Thermo Scientific™ F170S
111.10 EUR valido fino al 2024-11-30
Usa il codice promo "21651" per avere il tuo prezzo promozionale.
Descrizione
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, humans, fish, birds, and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue–derived inhibitors such as collagen, melanin and eumelanin (hair, skin), and myoglobin (muscle). The kit also includes DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.
Features
- Direct PCR reduces time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
- Specially engineered Phire DNA polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifica
• 2X Phire Tissue Direct PCR Master Mix, 2 x 1.25 mL • Dilution Buffer, 5 mL • DNARelease Additive, 3 x 100 μL • 25 μM Control Primer Mix, 40 μL • Nuclease-free water, 2 x 1.25 mL • 50 μg O'GeneRuler Express DNA Ladder Store at -20°C. |
|
Direct PCR | |
Fast | |
Direct PCR Master Mix | |
Genotyping, Hot-start PCR | |
Built-In Hot Start | |
Blunt | |
Tissue |
Medium | |
Phire Hot Start II DNA Polymerase | |
Yes | |
Wet or Dry Ice | |
2X | |
250 Reactions | |
SuperMix or Master Mix | |
DNA |
Sicurezza e movimentazione
- Phire Tissue Direct PCR Master Mix/DNARelease Additive
Avvertenza
- Pericolo
Categoria di pericolo
- Sensibilizzante delle vie respiratorie Categoria 1
Indicazioni di pericolo
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Consigli di prudenza
- P261-Evitare di respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P284-¢Quando la ventilazione del locale è insufficiente! indossare un apparecchio di protezione respiratoria.
- P342+P311-In caso di sintomi respiratori: contattare un CENTRO ANTIVELENI/un medico/ .
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P501-Smaltire il prodotto/recipiente in .
Informazioni supplementari
- MIXTURE LIST-Contiene: Proteinase, tritirachium album serine
For Research Use Only. Not for use in diagnostic procedures.