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Rat brain tissue lysate, Non-denatured; Abnova
Marca: Abnova L041W2.200ug
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Descrizione
- Tissue: Brain
- Host: Rat
- Lysis buffer: Modified RIPA lysis buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In modified RIPA lysis buffer
Western Blotting, Immunoprecipitation
Specifica
| Immunoprecipitation, Western Blot | |
| Whole tissue lysate (non-denatured) | |
| Tissue lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The lysate was adjusted to 5 mg/ml. | |
| 200 μg | |
| Rat | |
| Store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Brain | |
| Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. | |
| 12.5% SDS-PAGE Stained with Coomassie Blue. | |
| In modified RIPA Lysis Buffer. | |
| 5 mg/mL |
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