Restriction Enzymes

Restriction Enzymes
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Risultati della ricerca filtrata

Thermo Scientific™ FastDigest™ EcoRI
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | EcoR I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ BamHI
The BamHI restriction enzyme recognizes G^GATCC sites and cuts best at 37°C in its own unique buffer.
Concentrazione | 10 U/µl |
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Thermo Scientific™ EcoRI
The EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in its own unique buffer.
Concentrazione | 10 U/µl |
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Thermo Scientific™ FastDigest™ NcoI
The FastDigest NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Bsp19I).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Nco I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest™ HindIII
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Hind III |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest™ NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ HindIII
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.
Concentrazione | 10 U/μL |
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Thermo Scientific™ FastDigest™ BamHI
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | BamH I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest™ XhoI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Xho I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NheI
Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nhe I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ BglII
Cut at A^GATCT sites with BglII restriction enzyme, which performs best at 37°C in O buffer.
Concentrazione | 10 U/µl |
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Thermo Scientific™ FastDigest BglII
Cut at A^GATCT sites with FastDigest™ BglII restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | No |
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Product Type | Restriction Enzyme |
Enzyme | Bgl II |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Product Line | FastDigest™ |
Sito di taglio | CCC.GGG |
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Forma | Liquid |
Product Type | SmaI |
Da utilizzare con (applicazione) | >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI |
pH | 7.4 |
Componenti | 10X OPTIZYME™ Buffer 4 |
Tampone di conservazione | 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol |
Temperatura incubatore | 30°C |
Concentrazione | 10 U/μL |
Thermo Scientific™ Eco31I (BsaI)
The Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Eco31I (BsaI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Concentrazione | 10 U/μL |
Thermo Scientific™ FastDigest PacI
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.