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Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Antibiotic Resistance Bacterial | Ampicillin (AmpR) |
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Cloning Method | Restriction Enzyme⁄ MCS, Blunt PCR |
Da utilizzare con (applicazione) | PCR Cloning |
Vettore | pJET1.2/blunt |
Linea di prodotti | CloneJET |
Tipo di campione | PCR Products |
Formato | Kit |
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
Tecnica | Fluorescent Dye Terminator Sequencing |
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Da utilizzare con (apparecchiatura) | SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers |
Product Type | Sequencing Buffer |
Linea di prodotti | BigDye™ Terminator |
Formato | 12-well Strips,8-well Strip,96- or 384-well Plate |
Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.
Enzimi | DNA Ligase |
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Product Type | Rapid DNA Ligation Kit |
Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.
The DNA contained in the preparation has been lyophilized to maximize stability.The two hydrolytic enzymes used in this kit, shrimp alkaline phosphatase and exonuclease I, effectively remove excess dNTPs and primers.
This sequencing buffer is optimized for use with BigDye™ Terminator v1.1 and v3.1 Cycle Sequencing Kits.
Shipping Condition | Room Temperature |
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Tampone | Sequencing Buffer |
Content And Storage | Store at 2°C to 8°C. |
Forma | Liquid |
Da utilizzare con (applicazione) | Standard Sequencing |
Concentrazione | 5X |
Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.
Optimize cloning with Thermo Scientific™ pBR322 DNA, a 4361 bp double-stranded closed circular medium copy plasmid DNA.
The concentrated Running Buffer is used for electrophoresis on Applied Biosystems™3730 and 3730XL DNA Analyzers.
The GeneScan™ 1200 LIZ™ Size Standard is a fifth dye (LIZ™) labeled high density size standard for the reproducible sizing of fragment analysis samples.
A radio frequency identification tag is built into the frame for tracking critical run information, which is then reported on the software dashboard.
Invitrogen™ Platinum SuperFi Library Amplification Master Mix is a ready-to-use solution designed for amplification of next-generation sequencing (NGS) libraries.
Shipping Condition | Dry Ice |
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Forma | Liquid |
Reaction Format | SuperMix or Master Mix |
Product Type | Library Amplification Master Mix |
Hot Start | Built-In Hot Start |
Da utilizzare con (applicazione) | Sequencing |
Linea di prodotti | Collibri™ |
Durata del test | Standard |
Tipo di campione | DNA,RNA |
Sporgenza | Blunt |
Prestazioni PCR arricchito GC | High |
Fedeltà (rispetto a taq) | ≥100 |
Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.
Invitrogen™ Collibri™ Library Quantification Kit includes a ready-to-use master mix optimized for Illumina NGS library quantification and a library dilution buffer
Shipping Condition | Dry Ice |
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Da utilizzare con (apparecchiatura) | 7500 Fast System, 7900HT Fast System, QuantStudio™, ViiA™ 7 System, StepOne™ System, StepOnePlus™ System |
Content And Storage | Store at -20°C. |
Metodo di rilevamento | SYBR |
Product Type | Library Quantification Kit |
Polimerasi | Platinum™ II Taq Hot Start DNA Polymerase |
Da utilizzare con (applicazione) | Illumina NGS Library Quantification |
Linea di prodotti | Collibri™ |
Durata del test | Standard |
Tipo di campione | DNA,RNA |
PCR Method | qPCR |
Prestazioni PCR arricchito GC | Low |
Formato | Kit |