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BD Biosciences™ DAPI Solution (RUO)
Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.
Marca: BD Biosciences™ 564907
Dettagli aggiuntivi : Peso : 0.01000kg
Descrizione
- Nucleic acid stain that binds to A-T rich regions of DNA along the minor groove.
- DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells.
- Note, however, that high concentrations of the dye may still enter intact cells.
- Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry.
- When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm.
- DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA.
- Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.
Specifica
| 1.0 mg/mL | |
| Flow cytometry, bioimaging, immunofluorescence | |
| Avoid multiple freeze-thaws of product. The product should be kept undiluted at -20°C for long term storage, and it may be kept undiluted at 4°C for short term storage. |
| Nucleic acid stain | |
| 1 mg |