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Thermo Scientific™ Esente da DNasi I, RNasi
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Marca: Thermo Scientific™ EN0521
54.14 EUR valido fino al 2025-04-18
Usa il codice promo "24107" per avere il tuo prezzo promozionale.
Descrizione
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.
The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.
- In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
- In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides
Highlights
- Recombinant enzyme
- Purified from non-animal host with a lower level of intrinsic RNases
Applications
- Preparation of DNA-free RNA
- Removal of template DNA followingin vitrotranscription
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- DNA labeling by nick-translation in conjunction with DNA Polymerase I
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
Note
DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

Specifica
1 U/µl | |
Reaction Buffer | |
DNase I RNase Free |
DNase | |
1000U |
Suggerimenti di prodotto
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Certificati
Per poter ottenere i certificati è necessario indicare un numero di lotto. Per individuare il numero di lotto su ordini precedenti usi l'area dedicata allo stato degli ordini.
Numero di lotto | Tipo di certificato | Data | Catalog Number |
---|---|---|---|
2797506 | Certificato di analisi | 29/01/2025 | EN0521 |
2826726 | Certificato di analisi | 29/01/2025 | EN0521 |
2838992 | Certificato di analisi | 29/01/2025 | EN0521 |
2841107 | Certificato di analisi | 29/01/2025 | EN0521 |
2859648 | Certificato di analisi | 28/01/2025 | EN0521 |
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For Research Use Only. Not for use in diagnostic procedures.