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Thermo Scientific™ Esente da DNasi I, RNasi
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
Marca: Thermo Scientific™ EN0521
43.07 EUR valido fino al 2024-11-30
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Descrizione
Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.
The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ions.
- In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion
- In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides
Highlights
- Recombinant enzyme
- Purified from non-animal host with a lower level of intrinsic RNases
Applications
- Preparation of DNA-free RNA
- Removal of template DNA followingin vitrotranscription
- Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
- DNA labeling by nick-translation in conjunction with DNA Polymerase I
- Studies of DNA-protein interactions by DNase I, RNase-free footprinting
- Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used
Note
DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.
Specifica
| 1 U/µl | |
| Reaction Buffer | |
| DNase I RNase Free |
| DNase | |
| 1000U |
For Research Use Only. Not for use in diagnostic procedures.