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Cytiva HiLoad™ 16/600 Superdex™ 200 pg
Use for separating MAbs from dimers and contaminants. Cytiva HiLoad™ 16/600 Superdex™ 200 pg is ideal for high-resolution preparative gel filtration chromatography.
Marca: Cytiva 28-9893-35
Dettagli aggiuntivi : Peso : 0.96750kg
Includes:
- Dismantling tool
- Support screen
- Net ring (10μm)
- O-ring
- Stop plugs
- Instructions
Descrizione
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose. Dextran component determined the separation properties of these media. Steep selectivity curves give gppd resolving power for peptides and proteins in the molecular weight range Mr 10 000 600 000 (Superdex 200 pg).- Used for separating MAbs from dimers and contaminants
- Short run times with high recovery
- Medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions
- Separations can be scaled up to production levels
- 0.06 in. fittings to connect to AKTA design systems
- Theoretical plates: >13000m-1
- Flow rate: <1.6mL/min.
- Pressure max. (over the packed bed during operation): 3 bar [0.3MPa] (42psi)
- Media
- Average particle size: 34μm
- Matrix: composite of cross-linked agarose and dextran
- pH Stability working range: 3 to 12
- pH Stability cleaning range: 2 to 14
- Fractionation [Mr] globular proteins: 1 × 104-6 × 105
- Fractionation [Mr] dextrans: 1 × 103-1 × 105
- Shelf life from manufacture date: 5 years
- Chemical stability: all commonly used buffers, 1M NaOH, 0.1M HCl, 1M acetic acid, 8M urea, 6M guanidine HCl, 1% SDS, 70% ethanol, 30% iso-propanol, 30% acetonitrile
- Column
- Column I.D.: 0.62 in. (16mm)
- Inlet I.D.: 0.031 in. (0.8mm)
- Outlet I.D.: 0.031 in. (0.8mm)
- Chemical stability: resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol
- Column hardware made from borosilicate glass, polyamide, fuororubber, Ethylene Propylene Diene (EPDM), ETFE, Superpolyoxymethylene
- Column tube made from borosilicate glass
- Bed support made from Polypropylene (PP) (reinforced glass fiber), Polyamide (PA)
- Porosity: 10μm
Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids
Order Info
HiLoad columns are prepacked XK columns; Bed volume: 120mL; Bed dimensions: 0.62 x 23.62 in. (16 x 600mm); Sample volume: 5mL
Specifica
| <5 mL | |
| 4°C to 30°C, 20% Ethanol |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- HiLoad 16/600 Superdex 200 pg
Avvertenza
- Attenzione
Categoria di pericolo
- Liquido infiammabile Categoria 3
Indicazioni di pericolo
- H226-Liquido e vapori infiammabili.
Consigli di prudenza
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P210-Tenere lontano da fonti di calore, superfici riscaldate, scintille, fiamme e altre fonti di innesco. Vietato fumare.
- P241-Utilizzare impianti elettrici/di ventilazione/d'illuminazione/ a prova di esplosione.
- P303+P361+P353-IN CASO DI CONTATTO CON LA PELLE (o con i capelli): togliere immediatamente tutti gli indumenti contaminati. Sciacquare la pelle/fare una doccia.
- P235-Conservare in luogo fresco.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: ethanol