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Thermo Scientific™ Kit FITC, colorazioni fluorescenti e marcatura Pierce™
Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).
Marca: Thermo Scientific™ 46425
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Descrizione
Thermo Scientific™ FITC and Fluorescein Labeling Reagents and Kits are high-performance derivatives of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.Highlights:
- Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
Specifica
| 100 mg | |
| DMF (Dimethylformamide) | |
| Amine | |
| FITC (Fluorescein) | |
| Pierce™ | |
| 494 |
| FITC | |
| Store desiccated at -20°C, protect from moisture | |
| Isothiocyanate | |
| Classic Dyes | |
| 518 |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- FITC (5/6-fluorescein isothiocyanate), mixed isomer
Avvertenza
- Pericolo
Categoria di pericolo
- Sensibilizzante delle vie respiratorie Categoria 1
- Sensibilizzazione della pelle Categoria 1
Indicazioni di pericolo
- H317-Può provocare una reazione allergica cutanea.
- H334-Può provocare sintomi allergici o asmatici o difficoltà respiratorie se inalato.
Consigli di prudenza
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P304+P340-IN CASO DI INALAZIONE: trasportare l'infortunato all'aria aperta e mantenerlo a riposo in posizione che favorisca la respirazione.
- P310-Contattare immediatamente un CENTRO ANTIVELENI/un medico .
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Informazioni supplementari
- MIXTURE LIST-Contiene: 2-(6-hydroxy-3-oxo-(3H)-xanthen-9-yl) -5-isothiocyanatobenzoic acid
For Research Use Only. Not for use in diagnostic procedures.