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Thermo Scientific™ Reagenti di estrazione di proteine batteriche Pierce™ B-PER™
Efficient, gentle lysis and extraction of E.coli and other bacterial cells
Marca: Thermo Scientific™ 89821
303.78 EUR valido fino al 2024-11-30
Usa il codice promo "21651" per avere il tuo prezzo promozionale.
Codice prodotto. 15255743
Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Descrizione
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Specifica
| Detergente proprietario in tampone Tris 20 mM, pH 7.5, contenente isozima e nucleasi universale | |
| B-PER™ | |
| Bacterial Protein Extraction Reagent |
| Store at 4°C. | |
| 250 ml | |
| 250 mL |
For Research Use Only. Not for use in diagnostic procedures.