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Thermo Scientific™ Reagenti di estrazione di proteine batteriche Pierce™ B-PER™
Efficient, gentle lysis and extraction of E.coli and other bacterial cells
498.00€ - 850.00€
Specifica
Formulazione | Detergente proprietario in tampone Tris 20 mM, pH 7.5, contenente isozima e nucleasi universale |
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Product Type | Bacterial Protein Extraction Reagent |
Content And Storage | Store at 4°C. |
Product Line | B-PER™ |
Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Codice del prodotto | Marca | Quantità | Prezzo | Quantità e disponibilità | |||||
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Codice del prodotto | Marca | Quantità | Prezzo | Quantità e disponibilità | |||||
15255743
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Thermo Scientific™
89821 |
250 ml |
498.00€
250mL |
In stock
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15265743
|
Thermo Scientific™
89822 |
500 mL |
850.00€
500mL |
In stock
Eseguire il login per visualizzare lo stock disponibile |
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Descrizione
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Specifica
Detergente proprietario in tampone Tris 20 mM, pH 7.5, contenente isozima e nucleasi universale | |
Bacterial Protein Extraction Reagent | |
Store at 4°C. | |
B-PER™ |
For Research Use Only. Not for use in diagnostic procedures.
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