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Thermo Scientific™ Reagenti di estrazione di proteine batteriche Pierce™ B-PER™
Efficient, gentle lysis and extraction of E.coli and other bacterial cells
Marca: Thermo Scientific™ 89822
Dettagli aggiuntivi : Peso : 0.50000kg
Includes: Reagent packages are available in Tris and phosphate buffer formulations; Kits contain Tris-based B-PER Reagent plus lysozyme and DNase I enzymes.
Descrizione
- An easy-to-use cell lysis reagent that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
- All B-PER Reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay)
- This all-inclusive formulation combines the lysis reagent with lysozyme, and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria
- Depending on the particular application protease inhibitors, salts, reducing agents, denaturants, and chelating agents may be added to the reagent.
Features of the B-PER Bacterial Protein Extraction Reagent:
- Detergent in Tris buffer with Lysozyme and Universal Nuclease
- Improved cell lysis and DNA digestion, thereby releasing soluble proteins and reducing viscosity to increase yields
- Recovery of large molecular weight proteins (high MW proteins)
- Cost-effective equivalent to Merck Millipore Bugbuster™ Master Mix
- More effective than traditional sonication and typical homemade lysis buffers, many of which include detergents and components that interfere with downstream applications
Specifica
| Store at 4°C. | |
| Proprietary detergent in 20mM Tris buffer, pH 7.5, containing lysozyme and universal nuclease | |
| Bacterial Protein Extraction Reagent |
| B-PER™ | |
| 500 mL | |
| 500 mL |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- BPER Complete Bacterial Protein Extraction Reagent
Avvertenza
- Attenzione
Categoria di pericolo
- Lesione oculare grave/irritazione oculare Categoria 2
Indicazioni di pericolo
- H319-Provoca grave irritazione oculare.
Consigli di prudenza
- P264-Lavare accuratamente dopo l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
Informazioni supplementari
- MIXTURE LIST-Contiene: Poly(oxy-1,2-ethanediyl), .alpha.-[4-(1,1,3,3-tetramethyl butyl)phenyl]-.omega.-hydroxy
For Research Use Only. Not for use in diagnostic procedures.