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Thermo Scientific™ Soluzione di substrato TMB-Blotting 1-Step™
Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.
Marca: Thermo Scientific™ 34018
181.45 EUR valido fino al 2024-11-29
Usa il codice promo "21683" per avere il tuo prezzo promozionale.
Descrizione
Thermo Scientific™ Pierce TMB-Blotting 1-Step Solution is a ready-to-use solution of the blue TMB peroxidase (HRP) substrate that is specially formulated for chromogenic detection in Western blotting and immunohistochemistry methods.
TMB (3,3',5,5'-tetramethylbenzidine; MW = 240.4) is most often used as a soluble substrate to detect the activity of peroxidase-conjugated antibodies in ELISA. Pierce TMB-Blotting Solution is formulated to precipitate and localize the blue reaction product onto membrane surfaces or tissue samples at sites where the peroxidase-substrate reaction occurs. TMB-Blotting Substrate produces a very large signal-to-noise ratio, although its high sensitivity requires careful washing and blocking to avoid background staining. When performing immunohistochemical techniques, aqueous counterstains and mounting medium must be used.Highlights:
HRP substrate – for sensitive detection of horseradish peroxidase activity on solid media
Chromogenic – no special equipment needed for visualization; produces dark blue bands in Western blots; is not as susceptible to fading as AEC or 4-CN substrates
Ready-to-use – no organic solvents are required to dissolve; no dilution necessary for use
Complete – does not require other buffers or addition of hydrogen peroxide
Specifica
Substrate | |
Liquid | |
250 mL | |
Horseradish Peroxidase |
Colorimetric | |
1-Step™ | |
Horseradish Peroxidase | |
Upon receipt store product at 4°C. Product is shipped at ambient temperature. |
Sicurezza e movimentazione
- 1-Step TMB-Blotting, 250 mL
Avvertenza
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
- Tossicità specifica per organi bersaglio (esposizione singola) Categoria 2
Indicazioni di pericolo
- H302-Nocivo se ingerito.
- H371-Può provocare danni agli organi.
Consigli di prudenza
- P270-Non mangiare, né bere, né fumare durante l'uso.
- P260-Non respirare la polvere/i fumi/i gas/la nebbia/i vapori/gli aerosol.
- P264-Lavare accuratamente dopo l'uso.
- P301+P312-IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI/medico/
- P330-Sciacquare la bocca.
- P308+P311-In caso di esposizione o di possibile esposizione: contattare un CENTRO ANTIVELENI/un medico/.
Informazioni supplementari
- MIXTURE LIST-Contiene: Methyl alcohol
For Research Use Only. Not for use in diagnostic procedures.