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Thermo Scientific™ Kit GeneJET™ Plasmid Midiprep
Designed for large-scale isolation of high quality plasmid DNA from recombinant E.coli cultures.
Brand: Thermo Scientific™ K0482
This item is not returnable.
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Description
GeneJET Plasmid Midiprep Kit is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 25 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
Highlights
• Efficient—high yields up to 200 μg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified— >400 ng/μL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns
Applications
Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
• Transfection of robust cell lines
Specifications
| 400 ng/μL | |
| Spin Column | |
| Midi | |
| Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR | |
| 100-200 μg (Medium-Scale) Plasmid DNA | |
| Plasmid MidiPrep Kit | |
| Plasmid DNA |
| Spin Column | |
| Silica Column | |
| Bacteria | |
| Not High-throughput Compatible (Manual) | |
| GeneJET | |
| 100 Preps | |
| 60 min. |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- GeneJET Plasmid Midiprep Kit
Signal Word
- Attenzione
Hazard Category
- Tossicità acuta Categoria 4
- Lesione oculare grave/irritazione oculare Categoria 2
- Corrosione/irritazione della pelle Categoria 2
Hazard Statement
- H302-Nocivo se ingerito.
- H315-Provoca irritazione cutanea.
- H319-Provoca grave irritazione oculare.
Precautionary Statement
- P264-Lavare accuratamente dopo l'uso.
- P270-Non mangiare, né bere, né fumare durante l'uso.
- P280-Indossare guanti/indumenti protettivi/Proteggere gli occhi/il viso.
- P301+P310-IN CASO DI INGESTIONE: contattare immediatamente un CENTRO ANTIVELENI/un medico/
- P302+P352-IN CASO DI CONTATTO CON LA PELLE: lavare abbondantemente con acqua/.
- P305+P351+P338-IN CASO DI CONTATTO CON GLI OCCHI: sciacquare accuratamente per parecchi minuti. Togliere le eventuali lenti a contatto se è agevole farlo. Continuare a sciacquare.
- P330-Sciacquare la bocca.
- P332+P313-In caso di irritazione della pelle: consultare un medico.
- P337+P313-Se l'irritazione degli occhi persiste, consultare un medico.
- P362+P364-Togliere tutti gli indumenti contaminati e lavarli prima di indossarli nuovamente.
- P501b-Smaltire il prodotto/recipiente in conformità con le disposizioni locali/regionali/nazionali/internazionali.
Supplemental information
- MIXTURE LIST-Contiene: acetic acid, tritonX-100, guanidine hydrochloride
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Product Title
Thermo Scientific™ Kit GeneJET™ Plasmid Midiprep > 100 Preps
For Research Use Only. Not for use in diagnostic procedures.
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