Thermo Scientific™ Pierce™ Protein A IgG Plus Orientation Kit, 2 mL

Descrizione

Thermo Scientific™ Pierce IgG Plus Orientation Kits bind and crosslink antibody to Protein A or Protein G agarose resin, thereby creating a reusable affinity column for purification of antigens.

The immobilization method is called orientation because antibodies are first captured to the affinity resin through their Fc regions, which are the target binding sites of Protein A and Protein G ligands. Thus, the antibodies are bound with their antigen-binding sites oriented outward. DSS crosslinker reacts with primary amines (side chain of lysine residues) of the Protein A/G and affinity-bound antibody, thereby covalently and permanently attaching the proteins together.

Highlights:

• Contaminant-free antigen purification – enables antigen purification without contamination (co-elution) of antibody

• Covalent, leak-resistant antibody immobilization – high-capacity binding of IgG to Protein A or Protein G agarose, followed by efficient crosslinking with DSS crosslinker

• High-quality agarose resin – crosslinked 6% beaded agarose with recombinant Protein A or Protein G

• Gentle binding and crosslinking conditions – coupling antibody in standard PBS

• Oriented immobilization – binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance

• Complete kits – include all the reagents needed to immobilize the antibody

• Better than DMP – less antibody leaching than traditional orientation method with dimethyl pimelimidate crosslinker

Includes:

Protein A or G columns, disuccinimidyl suberate (DSS), PBS pack, blocking buffer, binding/wash buffer, elution buffer, and column accessories