Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

Improve sequence coverage and protein identification for mass spectrometry with high-quality, MS-grade proteases provided in stable liquid or lyophilized formats. Suitable for either in-solution or in-gel digestion workflows, each protease may be used individually or in combination to improve protein sequence coverage.

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Beta-mercaptoetanolo (bME, 2BME, 2-ME, b-mer), CAS 60-24-2 puro, liquido (equivalente a 14 M); agente riducente con tiolo per la scissione dei ponti disolfuro proteici (cistine, cisteine).

0.05% Trypsin and 0.5mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL

Methyl methanethiosulfonate (CAS 2949-92-0); reversibly blocks cysteines (sulfhydryl groups) to facilitate study of enzyme activation and protein functions.

Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

No-Weigh™ Format

Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Biotinylate protein amines (e.g., lysines) with a simple, short (13.5A), NHS-ester biotinylation reagent containing a membrane-permeable spacer arm for intracellular labeling.

Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).

Make sulfhydryl assay standards or perform protein refolding experiments using this pure, crystalline cysteine hydrochloride (Cys-HCl).

CAS: 9001-73-4 Sinonimo: EC 3.4.22.2

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Get rapid carboxymethylation of reduced cysteine residues with Thermo Scientific™ Pierce™ Iodoacetic Acid and Iodoacetamide.

Used for selective cleavage of proteins for amino acid sequence determination or peptide mapping

Fluorescence Dye 647-I with Phalloidin conjugate

MT(PEG)n: methyl and sulfhydryl compound with a 4-unit polyethylene glycol (PEG) spacer; methylate and pegylate quantum dots, gold surfaces, silver.

CA(PEG)n: pegylated amino acids of the form carboxy-PEG-amine; with 4 to 24 polyethylene glycol units; for surface- and molecule-pegylation applications.

Improve peptide digest quality and sensitivity of MS detection using thesw Thermo Scientific Digestion Kits for efficient and reproducible preparation of peptide digests.

Enhance aldehyde-hydrazide crosslinking efficiency for glycoprotein immobilization and conjugation with analine (CAS 62-53-3) and an optimized buffer system.

Biotinylate reduced cysteines (-SH groups) at high pH via irreversible thioether bonds with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent.

Biotinylate reduced cysteines (-SH groups) at high pH with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent containing polyethylene glycol for solubility.

Reliably label (iodinate) proteins and peptides with iodine-125 using glass test tubes that are pre-coated (activated) with iodination reagent.

Label and detect azide-tagged molecules for metabolic labeling (Staudinger ligation) for fluorescence assays with phosphine-activated fluorescent dyes.