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Thermo Scientific™ PCR Master Mix (2X)
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
Marca: Thermo Scientific™ K0171
83.20 EUR valido fino al 2024-12-20
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Descrizione
Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
- Convenient, ready-to-use mix
- Thermostable—half life is more than 40 min. at 95°C
- Generates PCR products with 3'-dA overhangs
- Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
- Routine PCR amplification of DNA fragments up to 5 kb
- High throughput PCR
- DNA labeling
Note
The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
Specifica
| • 2X PCR Master Mix, 4 x 1.25 mL • Nuclease-free water, 4 x 1.25 mL Store at -20°C. |
|
| Taq DNA Polymerase | |
| Miscela master per PCR (2X) | |
| 2X | |
| No | |
| 3'-A | |
| DNA |
| Low | |
| Standard | |
| Standard PCR | |
| 1X | |
| 200 Reactions | |
| SuperMix or Master Mix |
For Research Use Only. Not for use in diagnostic procedures.