Life Science Buffers

Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Fisher Bioreagents PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Tamponi di uso comune
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Fisher Bioreagents Compresse PBS, tampone fosfato salino, Fisher BioReagents
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
Thermo Scientific™ Dry-blend Buffered Packs
Our quality control ensures that every pack of dry-blend buffers yields consistent solutions without weighing or pH adjustment. Simply empty contents of one foil envelope pack into a beaker, add appropriate solvents, and stir to dissolve.

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Forma | Powder |
---|---|
Linea di prodotti | BupH™ |
Fisher Bioreagents Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
Sinonimo | TE |
---|---|
Nota nome | 1X Solution, pH 8.0 |
Pericolo per la salute 2 | CAUTION! |
Colore | Colorless |
Pericolo per la salute 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
Forma fisica | Liquid |
Conservazione consigliata | RT |
Filtered Through | Filtered through a 5-micron filter. |
DNasi | Esente da DNasi |
CAS | 7732-18-5 |
pH | 7.4 to 8.1 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | Tris-EDTA |
Grado | Biologia molecolare |
Fisher Bioreagents HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Agente tamponante molto diffuso
Peso formulazione | 238.197 |
---|---|
Sinonimo | N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid |
Note sul grado di purezza | DNase-, RNase- and Protease-Free |
Nota nome | 1M Solution, pH 7.3 |
Colore | Non definito |
Forma fisica | Liquido |
Conservazione consigliata | RT |
DNasi | Esente da DNasi |
Identificazione | Pass Test |
Absorbance | ≤0.01(0.1M solution) at 260nm,0.004 max. (0.1M solution) at 280nm |
Formula molecolare | C8H18N2O4S |
CAS | 7732-18-5 |
Indice di Merck | 15, 4689 |
pH | 7.2 to 7.5 |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | HEPES Buffer |
Grado | Biologia molecolare |
Concentrazione | 0.95 to 1.05 M |
Content And Storage | Room temperature |
---|---|
Forma | Liquid |
Punto di fusione | 0°C |
pH | 7.3 to 7.5 |
Tipo di imballaggio | Poly Cube |
Thermo Scientific PBS, 10X Solution, Molecular Biology Grade, Thermo Scientific Chemicals
For biological research. Thermo Scientific™ PBS, 10X Solution, Molecular Biology Grade is 0.2μm filtered and dispensed into durable, square nalgene bottles and upright carton.
Forma | Solution |
---|---|
pH | 7.4 |
Formulazione | 80.6 mM sodium phosphate, 19.4 mM potassium phosphate, 27 mM KCl and 1.37 M NaCl in high purity dH2O |
Nota nome | 100X Solution |
---|---|
Pericolo per la salute 2 | WARNING! |
Colore | Undesignated |
Pericolo per la salute 3 | Emergency Overview Irritating to eyes and skin. Wear personal protective equipment. Ensure adequate ventilation. Avoid contact with skin, eyes and clothing. Avoid ingestion and inhalation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Get medical attention immediately if symptoms occur. Do not induce vomiting. NFPA Health:2 Flammability:0 Instability:0 |
Forma fisica | Liquid |
CAS | 7732-18-5 |
Conservazione consigliata | RT |
Proteasi | Protease free |
Simbolo di conservazione sostanze chimiche | Gray |
Materiale o nome chimico | Tris-EDTA |
Filtered Through | Filtered through a 0.2-micron filter. |
Grado | Biologia molecolare |
DNasi | Esente da DNasi |
Colore | White |
---|---|
Content And Storage | Room temperature |
Forma | Powder |
Tipo di imballaggio | Poly Bottle |
Gibco™ DPBS, powder, no calcium, no magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15-30° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
Forma | Powder |
Osmolalità | 265 - 310 mOsm/kg |
Qualità di produzione | cGMP-compliant under the ISO 13485 standard |
Product Type | DPBS |
Classificazione | Animal Origin-Free |
pH | 7.2 to 7.7 |
Da utilizzare con (applicazione) | Mammalian Cell Culture |
Tipo di soluzione | Dulbecco's Phosphate Buffered Saline |
Additivo | No Sodium Pyruvate |
Linea di prodotti | Gibco™ |
Phenol Red Indicator | No Phenol Red |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.

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Thermo Scientific Chemicals TRIS-buffered saline (TBS, 20X) pH 7.4
CAS: 77-86-1 Formula molecolare: C4H11NO3 Molecular Weight (g/mol): 121.136 Numero MDL: MFCD00132476 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Numero MDL | MFCD00132476 |
---|---|
PubChem CID | 6503 |
Formula molecolare | C4H11NO3 |
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Thermo Scientific HEPES, 1M Solution, pH 7.3, Molecular Biology Grade, Ultrapure, Thermo Scientific Chemicals
CAS: 7365-45-9 Formula molecolare: C8H18N2O4S Molecular Weight (g/mol): 238.30 Numero MDL: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

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Numero MDL | MFCD00006158 |
---|---|
PubChem CID | 23831 |
Formula molecolare | C8H18N2O4S |
CAS | 7365-45-9 |
Molecular Weight (g/mol) | 238.30 |
ChEBI | CHEBI:42334 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |